7MZ2
Structure of human DNA polymerase beta complexed with dzA at N-1 of the template base paired with incoming dTTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0007 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.809, 80.181, 55.414 |
| Unit cell angles | 90.00, 107.56, 90.00 |
Refinement procedure
| Resolution | 19.865 - 2.088 |
| R-factor | 0.1989 |
| Rwork | 0.197 |
| R-free | 0.24280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bpx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.451 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 10.000 | 2.130 |
| High resolution limit [Å] | 2.088 | 5.630 | 2.090 |
| Rmerge | 0.116 | 0.041 | 0.473 |
| Number of reflections | 25175 | 1291 | 1276 |
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 99.9 | 99.9 | 99.9 |
| Redundancy | 5.6 | 5.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 14% to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5) |
