7MXV

Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' recessed-end DNA (ur)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	74.586, 74.586, 180.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.646 - 2.211
R-factor	0.2228
Rwork	0.221
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v05
RMSD bond length	0.004
RMSD bond angle	0.867
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.210	2.210
Rmerge	0.059	0.485
Number of reflections	26049	1111
<I/σ(I)>	38
Completeness [%]	98.5
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		290	100 mM sodium acetate, pH 7.0; 10 mM KCl; 2mM CaCl2; 20 mM MnCl2; 2-4% PEG 4000