7MXF
CD1c with antigen analogue 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9322 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.224, 71.363, 100.032 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.670 - 2.000 |
| R-factor | 0.2064 |
| Rwork | 0.204 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7mx4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.049 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.670 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.027 | 0.329 |
| Rmeas | 0.039 | 0.465 |
| Rpim | 0.027 | 0.329 |
| Number of reflections | 27417 | 2711 |
| <I/σ(I)> | 12.26 | 2.04 |
| Completeness [%] | 100.0 | 99.96 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.999 | 0.779 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 293 | 100 mM CHES, 1.05 M sodium citrate, 25 mM triglycine |
