7MX8

Crystal Structure of HPV L1-directed E7M03 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	55.716, 73.037, 125.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.490 - 2.020
R-factor	0.2101
Rwork	0.208
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5drx
RMSD bond length	0.007
RMSD bond angle	0.949
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	2.020	2.020
Number of reflections	34084	1659
<I/σ(I)>	15.7
Completeness [%]	99.3
Redundancy	4.5
CC(1/2)	0.974	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	1.5M Ammonium Sulfate, 0.1 M tris(pH 8.5), 12% glycerol