7MX8
Crystal Structure of HPV L1-directed E7M03 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.716, 73.037, 125.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.490 - 2.020 |
R-factor | 0.2101 |
Rwork | 0.208 |
R-free | 0.24590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5drx |
RMSD bond length | 0.007 |
RMSD bond angle | 0.949 |
Data reduction software | HKL-3000 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 2.020 | 2.020 |
Number of reflections | 34084 | 1659 |
<I/σ(I)> | 15.7 | |
Completeness [%] | 99.3 | |
Redundancy | 4.5 | |
CC(1/2) | 0.974 | 0.956 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 1.5M Ammonium Sulfate, 0.1 M tris(pH 8.5), 12% glycerol |