7MWM
Crystal structure of MBD2 with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.96863 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.511, 125.435, 40.318 |
Unit cell angles | 90.00, 117.50, 90.00 |
Refinement procedure
Resolution | 31.068 - 1.601 |
R-factor | 0.1867 |
Rwork | 0.186 |
R-free | 0.20610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6C2E |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER |
Refinement software | REFMAC (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.810 | 41.810 | 1.630 |
High resolution limit [Å] | 1.600 | 8.760 | 1.600 |
Rmerge | 0.053 | 0.023 | 1.085 |
Rmeas | 0.062 | 0.028 | 1.283 |
Rpim | 0.032 | 0.015 | 0.678 |
Total number of observations | 948 | 7514 | |
Number of reflections | 86004 | 267 | 2137 |
<I/σ(I)> | 14.9 | 45.2 | 1.1 |
Completeness [%] | 99.0 | 94.7 | 96.9 |
Redundancy | 3.8 | 3.6 | 3.5 |
CC(1/2) | 0.999 | 0.998 | 0.446 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 25% PEG3350, 0.2 M ammonium acetate, 0.1 M HEPES, pH 7.5 |