7MU8

Structure of the minimally glycosylated human CEACAM1 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	93.455, 93.455, 134.665
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.700 - 1.700
R-factor	0.2025
Rwork	0.202
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qxw
RMSD bond length	0.008
RMSD bond angle	1.023
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.730	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.072	2.009
Rmeas	0.076	2.103
Rpim	0.022	0.618
Number of reflections	25094	3588
<I/σ(I)>	12.88	1.11
Completeness [%]	99.8	99.89
Redundancy	11.2	11.5
CC(1/2)	0.999	0.494

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	100 mM MES, pH 6.5, 2M Ammonium sulfate