7MU2

Crystal Structure of WIPI2 in complex with W2IR of ATG16L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	I 1 2 1
Unit cell lengths	117.771, 49.075, 120.130
Unit cell angles	90.00, 95.86, 90.00

Refinement procedure

Resolution	38.940 - 1.850
R-factor	0.1837
Rwork	0.183
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4exv
RMSD bond length	0.007
RMSD bond angle	0.906
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.940	1.970
High resolution limit [Å]	1.850	1.850
Rmerge	0.050
Rmeas		0.031
Rpim	0.068
Number of reflections	55829	5022
<I/σ(I)>	6.9	2.7
Completeness [%]	98.5
Redundancy	3.3
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	22% w/v PEG 3,350, 2% v/v Tacsimate pH 7.0, 100mM Hepes pH 7.7