7MU2
Crystal Structure of WIPI2 in complex with W2IR of ATG16L1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 117.771, 49.075, 120.130 |
Unit cell angles | 90.00, 95.86, 90.00 |
Refinement procedure
Resolution | 38.940 - 1.850 |
R-factor | 0.1837 |
Rwork | 0.183 |
R-free | 0.21720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4exv |
RMSD bond length | 0.007 |
RMSD bond angle | 0.906 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.940 | 1.970 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.050 | |
Rmeas | 0.031 | |
Rpim | 0.068 | |
Number of reflections | 55829 | 5022 |
<I/σ(I)> | 6.9 | 2.7 |
Completeness [%] | 98.5 | |
Redundancy | 3.3 | |
CC(1/2) | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 22% w/v PEG 3,350, 2% v/v Tacsimate pH 7.0, 100mM Hepes pH 7.7 |