7MT4
Crystal structure of tryptophan Synthase in complex with F9, NH4+, pH7.8 - alpha aminoacrylate form - E(A-A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-08-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000014 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.086, 59.403, 67.343 |
| Unit cell angles | 90.00, 94.91, 90.00 |
Refinement procedure
| Resolution | 36.310 - 1.400 |
| Rwork | 0.177 |
| R-free | 0.20250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tjp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.150 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.270 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmeas | 0.363 | |
| Rpim | 0.146 | |
| Number of reflections | 198056 | 6836 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 98.7 | 95 |
| Redundancy | 12.1 | |
| CC(1/2) | 0.997 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 50 mM Bicine-NaOH pH 7.8, 10% PEG 8000, 2 mM spermine |
