7MRS

Zebrafish CNTN4 APPb complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-10
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	74.980, 88.190, 89.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.090 - 2.930
R-factor	0.2441
Rwork	0.240
R-free	0.28280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ktm 5e4s
RMSD bond length	0.003
RMSD bond angle	0.587
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.770	3.100
High resolution limit [Å]	2.930	2.930
Rmerge	0.165	1.163
Rmeas	0.174	1.227
Rpim	0.053	0.384
Number of reflections	13383	2111
<I/σ(I)>	9.8	2
Completeness [%]	100.0	100
Redundancy	10.4	10
CC(1/2)	0.991	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		277	75 mM NaCl, 10 mM Na-HEPES pH 7.5