7MRN

Mouse CNTN5 APP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	137.010, 137.010, 385.372
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.640 - 3.500
R-factor	0.248
Rwork	0.243
R-free	0.29090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ktm 5e52
RMSD bond length	0.002
RMSD bond angle	0.488
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.790	3.830
High resolution limit [Å]	3.500	3.500
Rmerge	0.216	1.323
Rmeas	0.229	1.396
Rpim	0.073	0.444
Number of reflections	17993	4233
<I/σ(I)>	8.8	1.9
Completeness [%]	100.0	100
Redundancy	9.7	9.8
CC(1/2)	0.983	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM Na-citrate pH 5.5, 10% Isopropanol, 25% (w/v) PEG 400