7MO3

Crystal Structure of the ZnF3 of Nucleoporin NUP153 in complex with Ran-GDP, resolution 2.05 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03317
Spacegroup name	P 1 21 1
Unit cell lengths	68.970, 62.550, 70.650
Unit cell angles	90.00, 105.12, 90.00

Refinement procedure

Resolution	29.390 - 2.050
R-factor	0.2052
Rwork	0.204
R-free	0.22820
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.390	29.390	2.120
High resolution limit [Å]	2.050	4.410	2.050
Rmeas	0.057	0.037	1.976
Rpim	0.021	0.014	0.725
Total number of observations		24187	24296
Number of reflections	66213	3522	3415
<I/σ(I)>	15.7	43.4	1.1
Completeness [%]	93.2	93.7	93.7
Redundancy	7.2	6.9	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	20% w/v PEG3350, 0.1 M Bis-Tris