7MO2
Crystal Structure of the ZnF2 of Nucleoporin NUP153 in complex with Ran-GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03317 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.089, 60.381, 79.426 |
Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
Resolution | 29.040 - 1.650 |
R-factor | 0.2125 |
Rwork | 0.211 |
R-free | 0.24100 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | AutoSol |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.040 | 29.040 | 1.680 |
High resolution limit [Å] | 1.650 | 9.030 | 1.650 |
Rmerge | 0.073 | 0.032 | 1.079 |
Rmeas | 0.079 | 0.035 | 1.186 |
Rpim | 0.030 | 0.013 | 0.481 |
Total number of observations | 2970 | 18540 | |
Number of reflections | 127956 | 426 | 3167 |
<I/σ(I)> | 13.8 | 41.6 | 1.5 |
Completeness [%] | 98.5 | 97.6 | 96.6 |
Redundancy | 6.8 | 7 | 5.9 |
CC(1/2) | 0.999 | 0.998 | 0.493 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 294 | 19% w/v PEG3350, 0.1 M Bis-Tris |