7MO1

Crystal Structure of the ZnF1 of Nucleoporin NUP153 in complex with Ran-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03317
Spacegroup name	P 21 21 2
Unit cell lengths	60.276, 79.619, 55.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.440 - 1.600
R-factor	0.1523
Rwork	0.151
R-free	0.17540
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.440	28.440	1.630
High resolution limit [Å]	1.600	8.750	1.600
Rmerge	0.078	0.032	1.717
Rmeas	0.081	0.033	1.790
Rpim	0.022	0.009	0.498
Total number of observations		3032	22144
Number of reflections	67816	265	1762
<I/σ(I)>	19	68.7	1.5
Completeness [%]	100.0	97.8	99.5
Redundancy	13	11.4	12.6
CC(1/2)	1.000	0.999	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294	22% w/v PEG3350, 0.1 M Bis-Tris