7MKK
Crystal structure of Drosophila Panoramix in complex with Sov NTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 127.389, 127.389, 96.012 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.829 - 2.500 |
R-factor | 0.2282 |
Rwork | 0.227 |
R-free | 0.25950 |
Structure solution method | SAD |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 16430 | 2329 |
<I/σ(I)> | 22.8 | |
Completeness [%] | 100.0 | |
Redundancy | 12.6 | |
CC(1/2) | 0.999 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M CHES pH 9.5, 30% (w/v) PEG 3000 |