7MI5
Asymmetrical PAM-Non PAM prespacer bound Cas4/Cas1/Cas2 complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS TALOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Phase Plate | VOLTA PHASE PLATE |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.57 |
| Software | cryoSPARC,RELION,EMAN |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
