7MGQ
AICAR transformylase/IMP cyclohydrolase (ATIC) is essential for de novo purine biosynthesis and infection by Cryptococcus neoformans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.841, 115.867, 111.823 |
| Unit cell angles | 90.00, 96.18, 90.00 |
Refinement procedure
| Resolution | 48.920 - 2.670 |
| R-factor | 0.2001 |
| Rwork | 0.199 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pkx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.638 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.920 | 2.770 |
| High resolution limit [Å] | 2.670 | 2.674 |
| Number of reflections | 65094 | 6258 |
| <I/σ(I)> | 71.97 | |
| Completeness [%] | 98.8 | |
| Redundancy | 2 | |
| CC(1/2) | 0.999 | 0.907 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M MgCl2, 20% w/v PEG 6000 and 0.2 M Tris base |
