7MFU
Crystal structure of synthetic nanobody (Sb14+Sb68) complexes with SARS-CoV-2 receptor binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.820, 83.050, 92.830 |
Unit cell angles | 90.00, 106.71, 90.00 |
Refinement procedure
Resolution | 40.270 - 1.700 |
R-factor | 0.1828 |
Rwork | 0.181 |
R-free | 0.21490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7klw |
RMSD bond length | 0.004 |
RMSD bond angle | 0.734 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.270 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.086 | 0.765 |
Rmeas | 0.104 | 0.925 |
Rpim | 0.057 | 0.510 |
Number of reflections | 105129 | 9993 |
<I/σ(I)> | 8.87 | 1.71 |
Completeness [%] | 98.4 | 93.8 |
Redundancy | 3.1 | 3.1 |
CC(1/2) | 0.995 | 0.640 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 8000, 0.1M Hepes pH 7.5 |