7MFO
X-ray structure of the L136 Aminotransferase from Acanthamoeba polyphaga mimivirus in the presence of TDP and PMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-23 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.885, 126.875, 206.365 |
| Unit cell angles | 90.00, 90.89, 90.00 |
Refinement procedure
| Resolution | 33.780 - 1.700 |
| R-factor | 0.1839 |
| Rwork | 0.182 |
| R-free | 0.22150 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.619 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 397722 | 18360 |
| <I/σ(I)> | 36.2 | 5 |
| Completeness [%] | 94.3 | 87.4 |
| Redundancy | 4.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | protein incubated with 1 mM PLP and 5 mM TDP-4-aminoquinovose. 11-14% PEG-8000, 200 mM KCl, 100 mM HEPES |
