7ME4

Structure of the extracellular WNT-binding module in Drosophila Ror2/Nrk

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9790
Spacegroup name	C 1 2 1
Unit cell lengths	95.704, 74.695, 61.552
Unit cell angles	90.00, 106.31, 90.00

Refinement procedure

Resolution	42.500 - 1.750
R-factor	0.1892
Rwork	0.187
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3HKL 1I71
RMSD bond length	0.013
RMSD bond angle	1.202
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX (dev_3915)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.074	42.501	1.840
High resolution limit [Å]	1.750	5.530	1.750
Rmerge		0.029	0.744
Rmeas	0.059	0.037	1.010
Rpim	0.036	0.023	0.678
Total number of observations	81880	2830	8391
Number of reflections	36932	1141	4747
<I/σ(I)>	9.1	26.3	1
Completeness [%]	88.5	85.4	78
Redundancy	2.2	2.5	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	294.15	3 mg/ml protein, 50 mM Bis-Tris propane (pH 5.0), 21% PEG 3350