7MD7

Crystal structure of the Thermus thermophilus 70S ribosome in complex with triphenylphosphonium analog of chloramphenicol CAM-C4-TPP and protein Y (YfiA) at 2.80A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	209.500, 448.640, 621.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	152.160 - 2.800
R-factor	0.2202
Rwork	0.218
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.003
RMSD bond angle	0.655
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	152.160	152.160	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.201	0.039	1.507
Rmeas	0.237	0.047	1.785
Total number of observations	4696602
Number of reflections	1382525	15550	101361
<I/σ(I)>	6.08	22.54	0.86
Completeness [%]	97.5	92.5	97.1
Redundancy	3.397	3.401	3.335
CC(1/2)	0.979	0.995	0.187

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME