7M9L
HIV-1 Protease WT (NL4-3) in Complex with LR4-15
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.07812 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.212, 59.055, 61.695 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.661 - 1.752 |
R-factor | 0.1788 |
Rwork | 0.177 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dgx |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.661 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.101 | 0.717 |
Number of reflections | 18787 | 1822 |
<I/σ(I)> | 25.8 | 3.3 |
Completeness [%] | 97.2 | |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23-24% (w/v) Ammonium Sulfate, 0.1M Bis-Tris-Methane-HCl Buffer pH 5.5 |