7M7N

Human DNA Pol eta with 2'-FA-ended primer and dAMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.444, 98.444, 82.105
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.310 - 1.310
R-factor	0.1863
Rwork	0.184
R-free	0.21030
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ecq
RMSD bond length	0.006
RMSD bond angle	0.870
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.330
High resolution limit [Å]	1.310	3.550	1.310
Rmerge	0.057	0.026	0.689
Total number of observations	413501
Number of reflections	104816	5271	4978
<I/σ(I)>	11.1
Completeness [%]	97.1	95.6	92.6
Redundancy	3.9	3.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	295	15% PEG 2000 MME, 100 mM MES