7M2I
Structural Snapshots of Intermediates in the Gating of a K+ Channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2019-06-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03 |
Spacegroup name | I 4 |
Unit cell lengths | 155.250, 155.250, 74.415 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.812 - 2.695 |
R-factor | 0.2305 |
Rwork | 0.228 |
R-free | 0.25050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4c |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.600 | 2.850 |
High resolution limit [Å] | 2.690 | 2.720 |
Number of reflections | 24336 | 3166 |
<I/σ(I)> | 8.1 | |
Completeness [%] | 100.0 | |
Redundancy | 6.8 | |
CC(1/2) | 0.990 | 0.320 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 50 mM MES, pH 6.25, 28% PEG400, 50 mM magnesium acetate |