7M1M

Crystal structure of Pseudomonas aeruginosa ClpP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	96.975, 97.118, 108.347
Unit cell angles	66.99, 85.93, 77.17

Refinement procedure

Resolution	48.540 - 2.600
R-factor	0.2078
Rwork	0.207
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qwd
Data reduction software	XSCALE
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.540	48.540	2.660
High resolution limit [Å]	2.600	11.610	2.600
Rmerge	0.131	0.044	0.557
Rmeas	0.156	0.054	0.692
Rpim	0.094
Number of reflections	103306	1190	7029
<I/σ(I)>	6.44	16.26	1.85
Completeness [%]	94.2	96.5	87.4
Redundancy	2.712	2.775	2.642
CC(1/2)	0.990	0.996	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M sodium malonate, 53.5% MPD