7M15
crystal structure of cj1430 in the presence of GDP-D-glycero-L-gluco-heptose, a GDP-D-glycero-4-keto-D-lyxo-heptose-3,5-epimerase from campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-05 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.409, 81.569, 109.453 |
| Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.1924 |
| Rwork | 0.190 |
| R-free | 0.23370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7m14 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.626 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 99769 | 13932 |
| <I/σ(I)> | 10.3 | 2.5 |
| Completeness [%] | 97.7 | 93.9 |
| Redundancy | 4.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 22-27% PEG-5000, 100 mM MOPS, 10 mM GDP-D-glycero-L-gluco-heptose |
