7M10
PHF2 PHD Domain Complexed with Peptide From N-terminus of VRK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 34.240, 63.717, 103.152 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.500 - 1.150 |
R-factor | 0.157 |
Rwork | 0.157 |
R-free | 0.16480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kqi |
RMSD bond length | 0.004 |
RMSD bond angle | 0.785 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.500 | 1.190 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.106 | 0.579 |
Rpim | 0.034 | 0.370 |
Number of reflections | 33230 | 991 |
<I/σ(I)> | 20.1 | 1.5 |
Completeness [%] | 81.5 | 24.7 |
Redundancy | 9.1 | 2.3 |
CC(1/2) | 1.000 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 292 | 3.5M Sodium Formate,100mM TRIS pH 8.2 |