Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 116.716, 116.716, 129.483 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.080 - 3.450 |
| R-factor | 0.1964 |
| Rwork | 0.195 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pp9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.774 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.080 | 3.575 |
| High resolution limit [Å] | 3.450 | 3.451 |
| Rmerge | 0.150 | 0.675 |
| Rmeas | 0.169 | 0.758 |
| Rpim | 0.075 | 0.338 |
| Number of reflections | 13509 | 1329 |
| <I/σ(I)> | 9.4 | 2.28 |
| Completeness [%] | 97.5 | 98.37 |
| Redundancy | 4.8 | |
| CC(1/2) | 0.992 | 0.748 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris 8.5, 200 mM Lithium Sulfate and 22% PEG 3,350 |
