7M0I
Crystal structure of a human metapneumovirus subtype B2 trimeric fusion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 114.530, 128.100, 431.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.690 - 2.810 |
R-factor | 0.2558 |
Rwork | 0.254 |
R-free | 0.29080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l1x |
RMSD bond length | 0.013 |
RMSD bond angle | 1.764 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.690 | 2.911 |
High resolution limit [Å] | 2.810 | 2.811 |
Number of reflections | 155097 | 1294 |
<I/σ(I)> | 6.5 | 0.77 |
Completeness [%] | 75.6 | |
Redundancy | 14.9 | 14.5 |
CC(1/2) | 0.973 | 0.227 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Tris pH 8.5, 2.0 M Ammonium sulfate |