7M05
CryoEM structure of PRMT5 bound to covalent PBM-site inhibitor BRD-6988
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.39 |
| Number of Particle | 956646 |
| Software | cisTEM,EPU,PHENIX |
| Symmetry | D2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
