7LZ8
Tubulin-RB3_SLD-TTL in complex with compound 5t
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.570, 155.041, 182.339 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.360 - 2.920 |
R-factor | 0.1858 |
Rwork | 0.184 |
R-free | 0.22760 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 6br1 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.617 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.085 | 0.869 |
Rpim | 0.048 | 0.506 |
Number of reflections | 64109 | 6296 |
<I/σ(I)> | 16.2 | 1.1 |
Completeness [%] | 98.7 | 98.7 |
Redundancy | 4.2 | 4.2 |
CC(1/2) | 0.994 | 0.465 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | PEG 4000, MES, glycerol, CaCl2, MgCl2, ethyl acetate |