7LZ5
Crystal structure of oncogenic KRAS Q61E GMPPCP-bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 94.413, 94.413, 119.326 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.680 - 1.500 |
| R-factor | 0.1648 |
| Rwork | 0.164 |
| R-free | 0.17910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vq2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.274 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 1.538 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.070 | 0.280 |
| Number of reflections | 32859 | 1624 |
| <I/σ(I)> | 38.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.6 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 3350, 0.2 M ammonium sulfate, 0.1 M HEPES |
