7LZ4
Crystal structure of A211D mutant of Protein Kinase A RIa subunit, a Carney Complex mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 176.786, 176.786, 345.503 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.249 - 4.155 |
R-factor | 0.2239 |
Rwork | 0.221 |
R-free | 0.26990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rgs |
RMSD bond length | 0.029 |
RMSD bond angle | 3.260 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.220 |
High resolution limit [Å] | 4.150 | 4.150 |
Rmerge | 0.180 | 0.460 |
Number of reflections | 30356 | 1504 |
<I/σ(I)> | 12.8 | 1.9 |
Completeness [%] | 99.3 | 100 |
Redundancy | 5.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295.5 | The protein was concentrated to 8 mg/mL, and crystallized in 2 uL hanging drops using the vapor diffusion method with 75 mM Sodium Acetate (pH 5.0), 2.0 M sodium formate, and four-fold molar excess cAMP at room temperature |