7LYW

Crystal structure of the HCMV pentamer-specific antibody 2-25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2017-08-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	180.870, 180.870, 138.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.080 - 2.510
Rwork	0.187
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n4j
RMSD bond length	0.011
RMSD bond angle	1.226
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3758)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.890	2.570
High resolution limit [Å]	2.510	2.510
Number of reflections	77232	7688
<I/σ(I)>	9.3	2
Completeness [%]	98.4
Redundancy	6.8
CC(1/2)	0.991	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	30% PEG 4000, 0.1M BIS-TRIS pH 6.5, 0.1 M calcium chloride, 0.1 M magnesium chloride