7LYW
Crystal structure of the HCMV pentamer-specific antibody 2-25
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2017-08-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 180.870, 180.870, 138.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.080 - 2.510 |
Rwork | 0.187 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n4j |
RMSD bond length | 0.011 |
RMSD bond angle | 1.226 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3758) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.890 | 2.570 |
High resolution limit [Å] | 2.510 | 2.510 |
Number of reflections | 77232 | 7688 |
<I/σ(I)> | 9.3 | 2 |
Completeness [%] | 98.4 | |
Redundancy | 6.8 | |
CC(1/2) | 0.991 | 0.522 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 30% PEG 4000, 0.1M BIS-TRIS pH 6.5, 0.1 M calcium chloride, 0.1 M magnesium chloride |