7LYF
Dengue virus RNA promoter stem-loop A fused with tRNA-scaffold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-07 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9785 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 30.924, 137.558, 336.877 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.688 - 3.400 |
R-factor | 0.2299 |
Rwork | 0.226 |
R-free | 0.26830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tn1 5ew4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.281 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.460 |
High resolution limit [Å] | 3.366 | 3.370 |
Number of reflections | 10308 | 10308 |
<I/σ(I)> | 37 | |
Completeness [%] | 97.2 | |
Redundancy | 7.1 | |
CC(1/2) | 0.991 | 0.636 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 100 mM sodium cacodylate pH 6.9, 9% (w/v) polyethylene glycol 8000, 200 mM KCl, and 100 mM magnesium acetate |