7LXR
Kinesin-like protein at 61F (Klp61f) bound to AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-29 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.692, 50.813, 93.344 |
| Unit cell angles | 78.53, 77.87, 73.20 |
Refinement procedure
| Resolution | 48.110 - 1.744 |
| Rwork | 0.185 |
| R-free | 0.22380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hqd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.953 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.110 | 1.840 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.057 | 0.272 |
| Number of reflections | 67986 | 9356 |
| <I/σ(I)> | 12.56 | 2.5 |
| Completeness [%] | 91.8 | |
| Redundancy | 3.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 10% ethylene glycol, 50mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (sodium HEPES) pH 7.5, 180mM NaI and 15-17% polyethylene glycol MW 3,350 |
