7LW8
Human Exonuclease 5 crystal structure in complex with a ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.1158 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 51.170, 83.980, 96.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.178 - 2.880 |
R-factor | 0.2073 |
Rwork | 0.205 |
R-free | 0.25860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lw7 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.481 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.180 | 48.180 | 3.040 |
High resolution limit [Å] | 2.880 | 9.110 | 2.880 |
Rmerge | 0.436 | 0.137 | 2.043 |
Rmeas | 0.455 | 0.145 | 2.126 |
Rpim | 0.126 | 0.045 | 0.585 |
Number of reflections | 9904 | 370 | 1407 |
<I/σ(I)> | 6.4 | 16.2 | 1.6 |
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 12.8 | 10.9 | 13 |
CC(1/2) | 0.986 | 0.994 | 0.620 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG 3350 and 0.2 M lithium acetate |