7LW7
Human Exonuclease 5 crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1158 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 50.818, 83.836, 95.965 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.983 - 2.500 |
R-factor | 0.1842 |
Rwork | 0.182 |
R-free | 0.22450 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.528 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.983 | 47.983 | 2.600 |
High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
Rmerge | 0.246 | 0.046 | 1.263 |
Rmeas | 0.267 | 0.051 | 1.373 |
Rpim | 0.104 | 0.021 | 0.531 |
Number of reflections | 14791 | 375 | 1617 |
<I/σ(I)> | 8.2 | 30 | 1.6 |
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 6.4 | 5.4 | 6.5 |
CC(1/2) | 0.988 | 0.998 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350 and 0.2 M lithium acetate |