7LW3
Structure of SARS-CoV-2 nsp16/nsp10 complex in presence of Cap-1 analog (m7GpppAmU) and SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0711 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 184.073, 184.073, 57.128 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.710 - 2.300 |
R-factor | 0.2238 |
Rwork | 0.222 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wks |
RMSD bond length | 0.027 |
RMSD bond angle | 2.323 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.040 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.089 | 1.800 |
Rmeas | 0.094 | |
Rpim | 0.029 | |
Number of reflections | 69833 | 4389 |
<I/σ(I)> | 15.2 | 0.9 |
Completeness [%] | 99.9 | 98.6 |
Redundancy | 10.3 | 10 |
CC(1/2) | 0.999 | 0.445 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 10% (v/v) propanol, 0.01 M MES/NaOH pH 6.0, 0.2 M calcium acetate |