7LVJ
CASP8 isoform G DED domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.984 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.912, 49.766, 52.271 |
| Unit cell angles | 90.00, 116.36, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.1993 |
| Rwork | 0.198 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Internal structure |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.980 | 35.980 | 1.580 |
| High resolution limit [Å] | 1.500 | 4.740 | 1.500 |
| Rmerge | 0.054 | 0.045 | 0.270 |
| Rmeas | 0.066 | 0.054 | 0.331 |
| Rpim | 0.037 | 0.030 | 0.188 |
| Total number of observations | 101116 | 3371 | 14492 |
| Number of reflections | 36268 | 1131 | 5309 |
| <I/σ(I)> | 10.6 | 22.5 | 2.7 |
| Completeness [%] | 97.3 | 92.3 | 98 |
| Redundancy | 2.8 | 3 | 2.7 |
| CC(1/2) | 0.996 | 0.995 | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 281 | 20% PEG 3350, 200mM Potassium Sodium Tartrate tetrahydrate |
