7LUO
N-terminus of Skp2 bound to Cyclin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 109.738, 109.738, 152.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.980 - 3.170 |
R-factor | 0.2357 |
Rwork | 0.234 |
R-free | 0.27720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fin |
Data reduction software | XDS (Jun 1, 2017) |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.17) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 109.740 | 109.740 | 3.390 |
High resolution limit [Å] | 3.170 | 8.970 | 3.170 |
Rmerge | 0.203 | 0.036 | 1.631 |
Rmeas | 0.217 | 0.039 | 1.737 |
Rpim | 0.073 | 0.014 | 0.581 |
Total number of observations | 142882 | 6203 | 26428 |
Number of reflections | 16322 | 817 | 2909 |
<I/σ(I)> | 10.3 | 42.4 | 1.4 |
Completeness [%] | 99.8 | 98.9 | 100 |
Redundancy | 8.8 | 7.6 | 9.1 |
CC(1/2) | 0.996 | 0.999 | 0.439 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 1.7 M Sodium-potassium phosphate |