7LUF
HSV1 polymerase ternary complex with dsDNA and PNU-183792
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 |
Unit cell lengths | 181.300, 181.300, 233.685 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.330 - 3.500 |
R-factor | 0.2268 |
Rwork | 0.226 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gv9 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 181.300 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 94834 | 4777 |
<I/σ(I)> | 8 | |
Completeness [%] | 100.0 | |
Redundancy | 6.6 | |
CC(1/2) | 0.997 | 0.702 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 283.15 | 0.1 M Na HEPES pH 6.8 and 0.4-0.7 M disodium tartrate |