7LT3
NHEJ Long-range synaptic complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | JEOL 3200FS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.6 |
| Number of Particle | 1119381 |
| Software | ISOLDE,EMAN,cryoSPARC,RELION,PHENIX,Appion,Gctf,CTFFIND,Coot |
| Symmetry | C2 |
| CTF correction | NONE |
