7LT2
Structure of a dsRNA-sensing cGAS-like receptor from the beetle Tribolium castaneum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-10-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.405, 70.443, 104.604 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.990 - 1.580 |
R-factor | 0.1868 |
Rwork | 0.186 |
R-free | 0.21510 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.660 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.990 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Rpim | 0.037 | 0.612 |
Number of reflections | 57602 | 2838 |
<I/σ(I)> | 9.6 | 1.1 |
Completeness [%] | 98.4 | |
Redundancy | 2.7 | |
CC(1/2) | 0.999 | 0.525 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.3 M potassium thiocyanate, 10-16% PEG3350 |