7LT2

Structure of a dsRNA-sensing cGAS-like receptor from the beetle Tribolium castaneum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-10-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	57.405, 70.443, 104.604
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.990 - 1.580
R-factor	0.1868
Rwork	0.186
R-free	0.21510
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.660
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.990	1.610
High resolution limit [Å]	1.580	1.580
Rpim	0.037	0.612
Number of reflections	57602	2838
<I/σ(I)>	9.6	1.1
Completeness [%]	98.4
Redundancy	2.7
CC(1/2)	0.999	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.3 M potassium thiocyanate, 10-16% PEG3350