EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8.4 |
| Number of Particle | 1766936 |
| Software | Gautomatch,Coot,EMAN,cryoSPARC,RELION,PHENIX,Gctf,CTFFIND,UCSF Chimera,ISOLDE |
| Symmetry | C1 |
| CTF correction | NONE |
