7LSD
Crystal structure of near-infrared fluorescent protein miRFP718nano
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-02-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.204, 103.198, 75.548 |
| Unit cell angles | 90.00, 125.75, 90.00 |
Refinement procedure
| Resolution | 29.840 - 1.700 |
| R-factor | 0.1582 |
| Rwork | 0.157 |
| R-free | 0.19580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mgh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.394 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.047 | 0.032 | 0.628 |
| Rmeas | 0.055 | 0.037 | 0.744 |
| Rpim | 0.029 | 0.020 | 0.395 |
| Total number of observations | 285750 | ||
| Number of reflections | 80892 | 8199 | 8019 |
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 96.6 | 96.6 | 96.4 |
| Redundancy | 3.5 | 3.5 | 3.4 |
| CC(1/2) | 0.998 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 12.6% PEG6000, 0.1 M lithium sulfate, 0.07 M citric acid, pH 3.5, 2.1% D-sorbitol |
