7LRQ
Crystal structure of human SFPQ/NONO heterodimer, conserved DBHS region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-04-04 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.128, 66.128, 203.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.040 - 2.300 |
| R-factor | 0.2598 |
| Rwork | 0.258 |
| R-free | 0.29920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wii |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.412 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.760 | 43.760 | 2.380 |
| High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
| Rmerge | 0.213 | 0.029 | 7.185 |
| Rmeas | 0.215 | 0.029 | 7.269 |
| Rpim | 0.028 | 0.004 | 1.094 |
| Total number of observations | 1299872 | 25715 | 97245 |
| Number of reflections | 23840 | 498 | 2273 |
| <I/σ(I)> | 26.1 | 123.1 | 1.4 |
| Completeness [%] | 100.0 | 99.3 | 99.9 |
| Redundancy | 54.5 | 51.6 | 42.8 |
| CC(1/2) | 1.000 | 1.000 | 0.528 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 22.5% PEG3350, 0.4 M MgCl2, 0.1 M HEPES pH 7.5 |
