7LRF

Netrin-1 in complex with SOS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-05-30
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54192
Spacegroup name	P 21 21 21
Unit cell lengths	75.105, 80.152, 241.702
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.357 - 3.210
R-factor	0.2291
Rwork	0.226
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ove
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2-3874_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.360	3.430
High resolution limit [Å]	3.208	3.210
Rmerge	0.138
Rmeas	0.172
Rpim	0.102
Number of reflections	24533	2351
<I/σ(I)>	9.3
Completeness [%]	99.3
Redundancy	8.3
CC(1/2)	0.995	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293.15	20% PEG 8000, 100 mM CHES