7LR2

Crystal structure of GH5_18 from Bifidobacterium longum subsp. longum ATCC 55813 in complex with GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	105.559, 143.486, 155.893
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.970 - 2.400
R-factor	0.1749
Rwork	0.172
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lr1
RMSD bond length	0.008
RMSD bond angle	0.897
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.090	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	92362	4443
<I/σ(I)>	8.5
Completeness [%]	99.3
Redundancy	13.5
CC(1/2)	0.996	0.681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.22 M ammonium phosphate monobasic