7LPY
Crystal structures and ribonuclease activity of the Flavivirus host factor ERI3 that is involved in viral RNA synthesis define the ERI subfamily of structure-specific 3-prime - 5-prime exoribonucleases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.555, 74.639, 96.709 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.320 - 1.850 |
R-factor | 0.1802 |
Rwork | 0.179 |
R-free | 0.20510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xri |
RMSD bond length | 0.005 |
RMSD bond angle | 0.794 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.320 | 1.960 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 240157 | 5775 |
<I/σ(I)> | 18.16 | |
Completeness [%] | 99.6 | |
Redundancy | 6.54 | |
CC(1/2) | 0.999 | 0.679 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 35% PEG 3350, 20% saturated KCl, 200 mM Imidazole / Malate buffer pH 8.0, 5mM MnCl2, 18mM AMP, 3.3 mM N-{2-[4-(thiophen-2-yl)-1H-imidazol-2-yl]ethyl}-2,3-dihydro-1,4-benzodioxine-2-carboxamide (from Life Chemicals) |