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7LPY

Crystal structures and ribonuclease activity of the Flavivirus host factor ERI3 that is involved in viral RNA synthesis define the ERI subfamily of structure-specific 3-prime - 5-prime exoribonucleases

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-2
Synchrotron siteSSRL
BeamlineBL9-2
Temperature [K]100
Detector technologyPIXEL
Collection date2021-01-11
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.97946
Spacegroup nameP 21 21 21
Unit cell lengths58.555, 74.639, 96.709
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution37.320 - 1.850
R-factor0.1802
Rwork0.179
R-free0.20510
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2xri
RMSD bond length0.005
RMSD bond angle0.794
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwarePHENIX (1.18.2_3874)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]37.3201.960
High resolution limit [Å]1.8501.850
Number of reflections2401575775
<I/σ(I)>18.16
Completeness [%]99.6
Redundancy6.54
CC(1/2)0.9990.679
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP29035% PEG 3350, 20% saturated KCl, 200 mM Imidazole / Malate buffer pH 8.0, 5mM MnCl2, 18mM AMP, 3.3 mM N-{2-[4-(thiophen-2-yl)-1H-imidazol-2-yl]ethyl}-2,3-dihydro-1,4-benzodioxine-2-carboxamide (from Life Chemicals)

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PDB entries from 2024-08-21

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