7LPY

Crystal structures and ribonuclease activity of the Flavivirus host factor ERI3 that is involved in viral RNA synthesis define the ERI subfamily of structure-specific 3-prime - 5-prime exoribonucleases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	58.555, 74.639, 96.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.320 - 1.850
R-factor	0.1802
Rwork	0.179
R-free	0.20510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xri
RMSD bond length	0.005
RMSD bond angle	0.794
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.320	1.960
High resolution limit [Å]	1.850	1.850
Number of reflections	240157	5775
<I/σ(I)>	18.16
Completeness [%]	99.6
Redundancy	6.54
CC(1/2)	0.999	0.679

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	35% PEG 3350, 20% saturated KCl, 200 mM Imidazole / Malate buffer pH 8.0, 5mM MnCl2, 18mM AMP, 3.3 mM N-{2-[4-(thiophen-2-yl)-1H-imidazol-2-yl]ethyl}-2,3-dihydro-1,4-benzodioxine-2-carboxamide (from Life Chemicals)