7LO9

RNA dodecamer containing a GNA A residue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9184
Spacegroup name	P 32
Unit cell lengths	29.370, 29.370, 133.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.760 - 1.780
R-factor	0.1959
Rwork	0.195
R-free	0.22590
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.534
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXDE
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.700	1.830
High resolution limit [Å]	1.780	1.780
Number of reflections	24620	1274
<I/σ(I)>	17.8
Completeness [%]	99.6
Redundancy	26.7
CC(1/2)	0.999	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	drop containing 0.5 mM oligonucleotide, 2.3 mM HoCl3, 40 mM NaCl, 5 mM spermine 4HCl, 20 mM Na cacodylate, pH 7.0 equilibrated against 40% MPD