7LO9
RNA dodecamer containing a GNA A residue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 32 |
Unit cell lengths | 29.370, 29.370, 133.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.760 - 1.780 |
R-factor | 0.1959 |
Rwork | 0.195 |
R-free | 0.22590 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.534 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.700 | 1.830 |
High resolution limit [Å] | 1.780 | 1.780 |
Number of reflections | 24620 | 1274 |
<I/σ(I)> | 17.8 | |
Completeness [%] | 99.6 | |
Redundancy | 26.7 | |
CC(1/2) | 0.999 | 0.792 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | drop containing 0.5 mM oligonucleotide, 2.3 mM HoCl3, 40 mM NaCl, 5 mM spermine 4HCl, 20 mM Na cacodylate, pH 7.0 equilibrated against 40% MPD |